Generator

Part:BBa_K1486020:Design

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-15)


rLuc[2]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When fused to another coding sequence, use a flexible linker. If fused to the N-terminal of another coding sequence, be careful to remove the stop codon.

Source

The part has been made by PCR amplification of the plasmid prLuc from Waldor Laboratory [http://waldorlab.bwh.harvard.edu/] used in the following paper: [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0043175].

References

S.K. Hatzios, S. Ringgaard, B. M. Davis, M. K. Waldor (2012, August 15). Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition